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Identification of ploidy levels of in vitro grown purple coneflower (Echinacea purpurea L.)

Authors:

N Dahanayake ,

University of Ruhuna, Kamburupitiya, LK
About N
Department of Agricultural Biology, Faculty of Agriculture
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Y Yue-Sheng

College of Life Sciences, South China, CN
About Y
Genetic Engineering Laboratory
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Abstract

Echinacea purpurea L. (Purple coneflower- Asteraceae) is one of the important medicinal herbs. In this study, three morphologically different types, haploid, diploid and tetraploid plantlets, were clearly identified using in vitro grown E. purpurea plantlets. Although all the rooted plants were almost the same height and possessed of almost the same number of leaves, diploid and tetraploid showed evidently sturdier roots while haploids had slimmer roots. Roots of diploids and tetraploids showed prominent differences; roots of tetraploids were shorter, thicker and darker in colour and with many lateral roots than those of diploids. The difference in the thickness of the roots could be distinguished as early as day 10-15, few days after growing out of adventitious roots. There were no significant differences in flowering time among haploids, diploids and tetraploids grown normally in the field and flowered within 5 months. There were no prominent morphological differences to be identified between E. purpurea haploids, diploids and tetraploids plant leaves either under in vitro or in vivo conditions. Size of stomata on leaves varied even within the same leaf. However, significant differences of the size of stomata could be found among haploids, diploids and tetraploids. The average length of stomata of diploid plants was 122.60 μm and that of tetraploid and haploid plants were 137.59 μm and 72.41 μm, respectively. Overall, tetraploids possessed longer and wider stomata. E. purpurea haploid has 11 chromosomes with smaller cells; diploid has 22 chromosomes and tetraploid has 44 chromosomes with comparatively larger cells than haploid and diploid plants.

DOI: http://dx.doi.org/10.4038/jur.v1i1.6156

Journal of the University of Ruhuna 2013 1(1): 17-21

DOI: http://doi.org/10.4038/jur.v1i1.6156
How to Cite: Dahanayake, N. & Yue-Sheng, Y., (2013). Identification of ploidy levels of in vitro grown purple coneflower (Echinacea purpurea L.). Journal of the University of Ruhuna. 1(1), pp.17–21. DOI: http://doi.org/10.4038/jur.v1i1.6156
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Published on 17 Oct 2013.
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