Water buffaloes contribute around 17% of the total milk production of the country. According to the department of census and statistics of Sri Lanka the buffalo population shows an increment during last decade and it has spread all over the country including southern province of Sri Lanka. However due to the present breed structure of the population a detailed study has to conduct to improve buffalo production through breeding. Selection of suitable animals plays an important role and genetic markers have a great potential. New sophisticated techniques which are based on DNA technology is more expensive to practice and more sophisticated facilities are essential. Therefore, conventional protein evaluating techniques based on polymorphism can be used as low cost methods. The study was conducted to identify the milk protein polymorphism of the buffalo milk and identify the relationships of the animals according to the milk protein polymorphism. Milk samples of four distinct groups of buffaloes (Surti cross bred, Local wild type, graded Murah and Local crossed) which had never meet one another were collected and used for protein separation by (NH4)2SO4. The separated proteins were run in Polyacrylamide gel using Sodium Dodecyl Sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) method and the polymorphism in the milk proteins were studied. Coomassi blue and silver staining methods were used for staining the gels. Milk proteins of the local wild type animals show a clear difference from other three groups of animals due to absence of a protein band of 87 kDa in 10 % SDS gel. There were differences in proteins between animal groups as well as within the same group. The most possible reason for that is the genetic diversity between the groups and within the group. For further studies on protein identification, sequencing of bands and studying the co- relation of genetic diversity and their morphological characters are essential.
Ranasinghe, K.K.K. et al., (2017). Milk protein polymorphism of buffaloes in southern Sri Lanka. Journal of the University of Ruhuna. 5(1-2), pp.14–25.
Ranasinghe KKK, Hirimuthugoda NY, Rajapakse RPVJ, Gunawardane A. Milk protein polymorphism of buffaloes in southern Sri Lanka. Journal of the University of Ruhuna. 2017;5(1-2):14–25.
Ranasinghe, K. K. K., Hirimuthugoda, N. Y., Rajapakse, R. P. V. J., & Gunawardane, A. (2017). Milk protein polymorphism of buffaloes in southern Sri Lanka. Journal of the University of Ruhuna, 5(1-2), 14–25.
Ranasinghe K.K.K. and others, ‘Milk Protein Polymorphism of Buffaloes in Southern Sri Lanka’  5 (14)